ABSTRACT
Objective To evaluate the clinical value of acetic acid with narrow-band imaging ( NBI ) and magnifying endoscopy ( ME ) on diagnosis of small colorectal polyps. Methods In this prospective study, 261 small colorectal polyps from 122 patients were observed by ME, NBI-ME, and acetic acid with NBI-ME, and then received endoscopic treatment. Endoscopic images were stored electronically and randomly allocated to 3 experts and 3 non-experts for diagnosis using Kudo pit pattern. The postoperative pathologic results acted as gold standard to evaluate the diagnostic accuracy of different endoscopic modes for small colorectal polyps. The image definition and interobserver agreement were compared among different endoscopic modes. Results The diagnostic accuracy of ME, NBI-ME, and acetic acid with NBI-ME for small colorectal polyps was 65. 5％ ( 171/261) , 90. 0％ ( 235/261) , and 94. 6％ ( 247/261) , respectively, in the experts group, and 57. 1％ ( 149/261) , 83. 1％ ( 217/261) , and 89. 3％ ( 233/261) , respectively, in the non-experts group. All experts and non-experts diagnosed small colorectal polyps more accurately by acetic acid with NBI-ME than by NBI-ME ( all P<0. 05 ) and ME ( all P<0. 001 ) . The image definition scores of acetic acid with NBI-ME in the experts group and non-experts group were significantly higher than those of NBI-ME and ME ( all P<0. 001) . The results of interobserver agreement showed that Kappa values (95％CI) of ME, NBI-ME, and acetic acid with NBI-ME diagnosis were 0. 578 (0. 508-0. 648), 0. 669 (0. 599-0. 739), and 0. 940 (0. 870-1. 010), respectively, for experts and 0. 476 (0. 406-0. 546), 0. 534 ( 0. 464-0. 604) , and 0. 830 ( 0. 760-0. 900 ) , respectively, for non-experts. Acetic acid with NBI-ME showed good interobserver agreement. Conclusion Acetic acid with NBI-ME has a higher diagnostic accuracy and good reproducibility for colorectal small polyps compared with ME and NBI-ME.
ABSTRACT
Objective To establish a HPLC method to determine the related substances of vitamin B6 for injection.Methods HPLC method was adopted with DiamonsilTM C18,5μm 250mm×4.6mm;the mobile phrase was length was 291nm.Results The calibration curve was linear in the concentration range of 600～1400μg/ml(r=0.9998).Conclusion The method is simple,rapid,accurate and reliable.As the supplement method of active pharmacopoeia,it can effectively control the quality of the product.
ABSTRACT
OBJECTIVE To analyze the incidence and related factors of the nosocomial infection in our hospital during a 3-year period.METHODS By using the retrospective method,all inpatient cases in our hospital from 2004 through 2006 were surveyed and analyzed.RESULTS Totally 44 759 inpatient cases were involved in the period,and 1653 cases with nosocomial infection occurred.The infection rate was 3.69%.The major sites of the infection were lower respiratory tract(45.12%),upper respiratory tract(19.12%)and urinary tract(13.77%).The most common pathogens were Klebsiella pneumonial(13.01%),Escherichia coli(11.53%),Pseudomonas aeruginosa(10.39%),and Acinetobacter baumannii(9.13%) in Gram-negative bacteria,and Staphylococcus aureus(5.02%) and S.epidermidis(3.42%) in Gram-positives.Candida albicans(21.8%) was the major isolate in Fungi.The infection rate in ICU was 20% higher than in other departments per year during 3 years.CONCLUSIONS Strengthening the medical knowledge of about nosocomial infection among employees,improving the environment quality of hospital,identifying pathogens correctly and surveying the resistance of antibiotic agents should be noted to control nosocomial infection.